An asymmetric PCR-ELISA typing assay for Citrus tristeza virus (CTV)
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چکیده
Typing of CTV based on coat protein (CP) gene has been approached in diverse ways. The basis for the assay focused on in this paper was presented some years ago and has been subject to several improvements since its appearance. Although the results obtained with this assay have been presented in diverse scientiic meetings, theses and technical seminars, so far no paper has explained the rationale of the method or detailed the protocol. This is presented here as well as the discussion of recent developments regarding the set of probes, hybridisation procedure and software for interpreting the results.
منابع مشابه
Development of a Multiplex RT-PCR Assay for Detection of the Causal Agents of Citrus Tristeza and Cachexia Diseases with Coamplification of Plant mRNA as an Internal Control
Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...
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A multiplex Taqman-based real-time reverse transcription (RT) polymerase chain reaction (PCR) assay was developed to identify potential severe strains of Citrus tristeza virus (CTV) and separate genotypes that react with the monoclonal antibody MCA13. Three strain-specific probes were developed using intergene sequences between the major and minor coat protein genes (CPi) in a multiplex reactio...
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تاریخ انتشار 2013